Quail Egg Image Analysis

We are working with the Childrens Cancer Therapy Development Center to help automate testing with quail eggs. This project aims to automate the process of checking eggs for current and future viability during treatment.

=Problem Definition=

Background
Preclinical research goes from in vitro studies that are inexpensive and not necessarily indicative of the disease to mouse studies that are better models but prohibitively expensive at scale. Chorioallantoic membrane (CAM) assays utilizing Japanese quail (Coturnix Japonica) are a cost-effective screening method to precede murine xenografts. To increase the throughput of CAM assays, an optimized platform has been built for processing 100-200 quail a week to validate one or more in vitro hit per week. Concordance and divergence from in vitro data and concordance with murine in vivo studies is demonstrated, suggesting a role for ex ovo studies in the validation of new anticancer agents.

Automatic detection of shell-free quail embryos that will not be viable is needed before tumor cell engraftment and drug testing are done. The Egg Buddy MK2 digital heart monitor provides proof of concept that EKG is possible.

Deliverables
Our goal is to create a device that can determine viability in a dark room environment. The device will consist of a camera and additional sensors feeding input to a driving program. This program will constantly assess specified metrics and output live results of the eggs viability. These results will be displayed via a multi-platform GUI application.

Specifications
The device should determine the following metrics by a combination of touch-free or minimally-invasive, aseptic electrodes. Imaging can be taken from above or below using visible or near-infrared light. Nothing should touch the internal components of the plate if possible. Components should have minimal luminescence or light-scattering.
 * Heart rate
 * Oximetry (oxygen saturation)
 * CAM blood vessel area
 * Branch point count

The device should keep its environment within the following parameters to support normal incubation of Coturnix Japonica:

=Design Considerations=

Hardware
The enclosure should contain a camera for imaging and additional sensors for tracking metrics such as heart rate, as well as environmental variables such as humidity and temperature. A system on a chip (SOC) should be mounted externally and connected to all sensors. A driver program connecting to this SOC should be able to run on various user devices. This connection can be made either by physical connection or a wireless protocol, such as Bluetooth.

Software
Vessel area and branch point count will be handled by NASA licensed software: Vessel Genration Analysis, (VESGEN). Receiving input for heart rate and oxygen saturation may require additional software, running on the SOC or driver program on a separate system. A driver program will run on the separate system, handling the processing and UI. This program should be multi-platform, running on Windows, OSX, and potentially Linux. Inputs should all be received by the SOC and then sent to the driver for processing and display.

This software should display all pertinent gathered or processed information. It should provide the status of each egg's viability in an intuitive and user-friendly form. It may also provide projections for future viability. =Project Learning=

=Final Design=

=Validation=

=Team Members=

=Additional Documentation=

Project Schedule

Meeting Minutes

Presentations


 * Meet The Team
 * Snapshot I