Atmospheric Microbial Sampling System

The goal of the project is to aseptically collect microbial samples from the jet stream using a simple and efficient sampling device.

=Problem Definition= Little research or information is known about the microbial life in the jet stream. For the purpose of the project, a sampling system will be developed in order to collect microbial samples while being contaminant free for further analysis.

Background
Studies speculate that microorganisms, some being pathogenic, can travel through the jet stream. In order to learn more about the atmospheric microorganism content, a method of sampling must be developed.

Deliverables
Once completed the sampling system will aseptically collect microbial life for classification of the microbiological taxa of the stratosphere. As well as providing records and data of temperature, humidity, and pressures at different altitudes.

Specifications
This sampling system is intended to collect microorganisms from the jet stream. The system design must keep sample collectors sterile before the launch and must prevent any contamination after the flight. Control systems of the device will be programmed to read the temperature, humidity, and pressure of the surrounding environment through sensors as the system ascends higher into the stratosphere. The determined launch platform is a high altitude balloon along with a parachute system for the descent. Samples collected from successful launches will be PCR replicated and DNA sequenced for exact microbial classification.

=Design Considerations= Both images below illustrate 3-D images of the proposed sampling system design. The system itself will be composed of 3 main pieces: The Impactor(bullet shaped object), collection cylinder, and collection media base plate. To the left is the initial drawing of the sampling system design. The geometry of the device resembles that of a bullet, but the shape itself aids in the collection of microbial samples. The inside upper portion of the impactor is funnel shaped in hopes of guiding airflow towards the collection media and increasing optimal microbial sampling. Small openings all along the device will act as vents to allow air flow and circulation. Figure 2 labels measurements for the sampling system. The system as a whole will be fair sized for all components included.

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=Additional Documentation=

Project Schedule



Meeting Minutes



Client Interview